Study on the fluorescent spectral characteristics of milk powder and melamine

YANG Cheng-fang; LI Lei; ZHANG Feng; WANG Dao-guang; LIU Cheng; LIU Ying

doi:10.3969/j.issn.1001-3806.2010.02.014

In order to obtain the steady state fluorescent and synchronous fluorescent spectral characteristics of milk powder, melamine and the mixture of milk powder-melamine respectively, UV light of 280nm was chosen as the excitation light to irradiate milk powder-water solution and the emission peak was at 330nm in the fluorescent spectrum. The constant-wavelength Δλ was used in synchronous fluorescence spectrometry. The experiment results reveal while changing the value of DA,the synchronous fluorescent intensity altered, and it reached maximum when Δλ=60nm. Scanning the synchronous fluorescence of different milk powder-water solutions with Δλ=60nm, the fluorescent intensity changed and the relationship between the fluorescence intensity and the concentration of the milk powder-water solution was linear. When Δλ=38nm, the synchronous fluorescent spectra of milk powder, melamine and the mixture of melamine and milk powder were obtained. The experiment results indicated that the synchronous fluorescent peak of milk powder was at 283nm, for melamine,it was at 319nm.In the milk powder-melamine solutions,there were two peaks of 283nm for milk powder and 319nm for melamine, so that whether there was melamine in the milk powder can be determined.The research may contribute to the research of the characteristics of melamine and its derivatives and the detection of melamine in the milk.

Study on the fluorescent spectral characteristics of milk powder and melamine

Corresponding author: LIU Ying, liuying70@126.com

1. College of Physics & Electronic Engineering, Xuzhou Normal University, Xuzhou 221116, China

Received Date: 2009-01-19

Accepted Date: 2009-03-13

Available Online: 2010-03-25

Abstract: In order to obtain the steady state fluorescent and synchronous fluorescent spectral characteristics of milk powder, melamine and the mixture of milk powder-melamine respectively, UV light of 280nm was chosen as the excitation light to irradiate milk powder-water solution and the emission peak was at 330nm in the fluorescent spectrum. The constant-wavelength Δλ was used in synchronous fluorescence spectrometry. The experiment results reveal while changing the value of DA,the synchronous fluorescent intensity altered, and it reached maximum when Δλ=60nm. Scanning the synchronous fluorescence of different milk powder-water solutions with Δλ=60nm, the fluorescent intensity changed and the relationship between the fluorescence intensity and the concentration of the milk powder-water solution was linear. When Δλ=38nm, the synchronous fluorescent spectra of milk powder, melamine and the mixture of melamine and milk powder were obtained. The experiment results indicated that the synchronous fluorescent peak of milk powder was at 283nm, for melamine,it was at 319nm.In the milk powder-melamine solutions,there were two peaks of 283nm for milk powder and 319nm for melamine, so that whether there was melamine in the milk powder can be determined.The research may contribute to the research of the characteristics of melamine and its derivatives and the detection of melamine in the milk.

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Reference (13)

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Study on the fluorescent spectral characteristics of milk powder and melamine

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