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Volume 28 Issue 5
Sep.  2013
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Ultra-trace analysis of biological molecules in water by means of the resonance Raman spectra in liquid-core optical fiber

  • Received Date: 2003-09-04
    Accepted Date: 2004-04-13
  • The study on detecting,identifying and controlling the biological molecules in water is profound and significant project.We used the means of the resonance Raman spectra in the liquid-core optical fiber,and the intensity of the Raman spectra was enhanced 109 times.β-carotene was dissolved in organic solution(pyridine and water),poured into a number of hollow-core capillary made up of liquid-core optical fiber.The laser wavelength is 514.5nm.The laser power is 30mW.By this means we obtained the resonance Raman spectra of β-carotene with the concentration of 10-7mol/L~10-9 mol/L.This means provided an experiment technique for trace analysis of biological molecules in water.
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  • [1] MIZUNO M,TAHARA T.Novel resonance Raman enhancement of local structure around solvated electrons in water[J].J Phys Chem,2001,105(39):8823~8826.[2] PEMBERTON R S,SHERVELL H F.Raman spectroscopy study of complex formation between P-cresol and propionitrile[J].J Raman Spectr,1995,26:373~380.[3] SINGH S,KRUEGER P J.Raman spectral studies of aqueous solutions of non-electrolytes:dimethylsulfoxide,acetone and acetonitrile[J].J Raman Spectr,1982,13(2):178~188.[4] ALTKORN R,KOEV I,PELLETIER M J.Raman performancecharacteristics of teflon AF 2400 liquid-core optical fiber sample cell[J].Appl Spectr,1999,53(10):1169~1176.[5] ALTKORN R,KOEV I,van DUYNE R P et al.Low-loss liquid-core optical fiber for low-refractive-Index liquids:fabrication,characterization,and application in Raman spectroscopy[J].Appl Opt,1997,36(34):8992~8998.[6] TSUNODA K I,NOMURA A,YAMADA J et al.The possibility of signal enhancement in liquid absorption spectrometry with a long capillary cell utilizing successive total reflection at the outer cell surface[J].Appl Spectr,1989,43(1):49~55.[7] 郑顺旋.激光喇曼光谱学[M].上海:上海科学技术出版社,1985.16~143.[8] 麦克黑尔 J L.分子光谱[M].北京:科学技术出版社,2003.364~406.[9] LI Z W,LI J,GAO Sh Q.Resonance Raman spectrum of β-carotene in liquid-core optical fiber[J].J A P,1998,37(4):1889~1891.[10] LI Z W,GAO Sh Q,SUN X et al.Effect of optical fiber loss on resonance Raman spectra of sample with low concentration[J].Spectr Letter,2001,34(5):569~578.[11] 里佐威,高淑琴,孙成林 et al.低浓度样品喇曼光谱的实验研究[J].分析化学,2000,28(2):1512~1515.

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通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Ultra-trace analysis of biological molecules in water by means of the resonance Raman spectra in liquid-core optical fiber

  • 1. College of Physics, Jilin University, Changchun 130023, China;
  • 2. Changchun Institute of Applied Chemistry, the Chinese Academy of Sciences, Changchun 130022, China

Abstract: The study on detecting,identifying and controlling the biological molecules in water is profound and significant project.We used the means of the resonance Raman spectra in the liquid-core optical fiber,and the intensity of the Raman spectra was enhanced 109 times.β-carotene was dissolved in organic solution(pyridine and water),poured into a number of hollow-core capillary made up of liquid-core optical fiber.The laser wavelength is 514.5nm.The laser power is 30mW.By this means we obtained the resonance Raman spectra of β-carotene with the concentration of 10-7mol/L~10-9 mol/L.This means provided an experiment technique for trace analysis of biological molecules in water.

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