Abstract: In order to explore the welding particularity of titanium alloy and stainless steel dissimilar metal and improve their welding performance, a new welding method of filling layer brass between titanium alloy and stainless steel was adopted. ANSYS finite element analysis software was applied. After theoretical analysis and experimental verification, it was concluded that the reasonable thickness of filling layer brass should be about 0.5mm~0.7mm. And based on simulation results, titanium steel dissimilar metal welding with the filling brass layer of thickness 0.5mm~0.7mm was used in welding experiment. Hardness, tensile test and scanning electron microscope (SEM) observation were carried out on the welded specimens. The results show that, when the thickness of the filler layer brass is 0.6mm, laser welding samples of Ti alloy and stainless steel has good weld morphology and mechanical properties.