Abstract: In order to use colored calcite crystal suitably,chemical composition of lilac color calcite crystal and yellowish calcite crystal was tested with ALR 9400 X-ray fluorescence spectrometer and X-ray diffractometer respectively.Transmitted spectra of yellowish calcite crystal were measured with spectrophotometer.It is found that the yellowish calcite crystal contains trace element Mg and Sr,and the lilac color calcite crystal contains trace element Mn,Sr and Mg.Extinction ratio of colored calcite crystal polarizer can reach 10-6.The results indicate that the polarizer can be used in optical measurement systems which don't need extreme high precision.