Abstract: In order to implement rapid quantitative phase measurement and imaging for transparent biological tissues, phase grating and other phase objects, based on the principle of in-line interferometry and phase contrast interferometry, an experimental system for fast quantitative measurement and imaging of phase objects was established. The principle of quantitative imaging was analyzed and the corresponding phase extraction and recovery procedures were designed. By shooting one or two phase contrast interferograms, the quantitative phase imagings for phase grating, water microdroplet and other phase objects with small phase variation were realized by the proposed method. A corresponding partition differential phase unwrapping algorithm was designed and the quantitative phase measurement and imaging of micro lens with phase value over π was achieved. The results show that the relationship between phase distribution of the obtained holographic phase grating measured by the system and phase amplitude variation of weak ultrasonic standing wave grating under different ultrasonic driving voltages is basically consistent with the results obtained from other methods. The experimental measurement of micro lens is compared with the theoretical value, and the absolute error is about 0.03μm. This system has certain feasibility, adaptability and potential applications on fast measurement and imaging of transparent phase objects such as biological cells and tissues.