Analization of blood cells fluorescence spectra induced by LED at the wavelength of 407nm
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摘要: 用光栅光谱仪获得了波长为407nm(Δλ1/2≈18nm)的LED激发的健康小白鼠的全血、红细胞和血红蛋白的荧光光谱,并对其产生机理和谱线特性进行了研究。实验结果和理论分析表明,407nm的LED诱导血液发出的荧光量子转换效率可达90%以上,且主要是由血液中红细胞上的荧光团产生的;溶血的红细胞产生荧光的量子产额明显较低,光谱特征也发生明显变化,且红细胞和血红蛋白的荧光光谱有较大的差异;血红蛋白的荧光量子产额将随浓度发生明显变化。Abstract: The fluorescence spectra for whole blood,erythrocyte and hemoglobin from healthy and fresh experimental mice under the wavelength of 407nm (Δλ1/2≈18nm) excitation are measured by a grating spectrometer,and their emitting mechanism and spectral characteristic are investigated. The experimental results and theoretic analization about it are presented in this paper. It shows that the blood or erythrocyte emitting fluorescence quantum yield efficiency under 407nm-LED excitation may reach above 90%,the blood fluorescence mainly result from fluorophores of the erythrocyte,but fluorescence spectra of hemoglobin by 407nm-LED excitation are obviously different from that of erythrocyte,and the fluorescence quantum yield of cracked erythrocyte remarkably declines and its spectral profiles are being distorted with the consistency of the cracked erythrocyte increasing.
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Keywords:
- fluorescence spectrum /
- LED /
- fluorophores /
- erythrocyte
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