Abstract: In order to suppress speckle noise in laser imaging, a laser microscopic speckle analysis and evaluation system was built by using the gray level co-occurrence matrix. In this system, a 405nm wavelength laser was used as light source of the microscopic system and the vibration of an sample driven by a voice coil motor through voltage was used to control the size of laser speckle noise. At the same time, a CCD image sensor was used to collect laser speckle noise under different driving voltages. Through analyzing the characteristic parameters such as angular second moment, contrast, entropy and inverse difference moment of laser speckle noise, the characteristics of laser speckle noise were appropriately characterized. The results show that the gray level co-occurrence matrix method can be used to evaluate the speckle in laser microscopic imaging.